Mutations affecting thymus organogenesis in Medaka, Oryzias latipes

نویسندگان

  • Norimasa Iwanami
  • Yousuke Takahama
  • Sanae Kunimatsu
  • Jie Li
  • Rie Takei
  • Yuko Ishikura
  • Hiroshi Suwa
  • Katsutoshi Niwa
  • Takao Sasado
  • Chikako Morinaga
  • Akihito Yasuoka
  • Tomonori Deguchi
  • Yukihiro Hirose
  • Hiroki Yoda
  • Thorsten Henrich
  • Osamu Ohara
  • Hisato Kondoh
  • Makoto Furutani-Seiki
چکیده

The thymus is an organ for T lymphocyte maturation and is indispensable for the establishment of a highly developed immune system in vertebrates. In order to genetically dissect thymus organogenesis, we carried out a large-scale mutagenesis screening for Medaka mutations affecting recombination activating gene 1 (rag1) expression in the developing thymus. We identified 24 mutations, defining at least 13 genes, which led to a marked reduction of rag1 expression in the thymus. As thymus development depends on pharyngeal arches, we classified those mutations into three classes according to the defects in the pharyngeal arches. Class 1 mutants had no or slight morphological abnormalities in the pharyngeal arches, implying that the mutations may include defects in such thymus-specific events as lymphocyte development and thymic epithelial cell maturation. Class 2 mutants had abnormally shaped pharyngeal arches. Class 3 mutants showed severely attenuated pharyngeal arch development. In Class 2 and Class 3 mutants, the defects in thymus development may be due to abnormal pharyngeal arch development. Those mutations are expected to be useful for identifying the molecular mechanisms underlying thymus organogenesis.

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عنوان ژورنال:
  • Mechanisms of Development

دوره 121  شماره 

صفحات  -

تاریخ انتشار 2004